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验证数据展示

  • FC experiment of human PBMCs using Biotin-65616

    FC experiment of human PBMCs using Biotin-65616

    1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a RecAb (Biotin-65616, Clone:26IC) and CoraLite®488-conjugated streptavidin, or unstained cells. Cells were co-stained with APC Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using Biotin-65616

    FC experiment of human PBMCs using Biotin-65616

    1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a RecAb (Biotin-65616, Clone:26IC)(red) and CoraLite®488-conjugated streptavidin, or unstained cells(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Biotin Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody

CD14 Uni-rAbTM Recombinant Antibody for FC
Cat No. Biotin-65616

产品说明书

CloneNo. 26IC

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 26IC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-65616 targets CD14 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD14 molecule
别名 Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 26IC
计算分子量 375 aa, 40 kDa
GenBank蛋白编号BC010507
基因名称 CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
偶联类型 Biotin
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
FC protocol for Biotin CD14 antibody Biotin-65616Download protocol
Standard Protocols
Click here to view our Standard Protocols