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验证数据展示

  • WB analysis using 31339-1-AP

    WB analysis using 31339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31339-1-AP (CD141/Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 31339-1-AP

    IF Staining of HUVEC using 31339-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CD141/Thrombomodulin antibody (31339-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

CD141/Thrombomodulin Polyclonal antibody

CD141/Thrombomodulin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31339-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

THBD, Thrombomodulin, TM, THRM, Fetomodulin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, THP-1 cells
Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD141/Thrombomodulin Ready-To-Use IHC Kit. CD141/Thrombomodulin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31339-1-AP targets CD141/Thrombomodulin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CD141/Thrombomodulin fusion protein Eg0944 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thrombomodulin
Synonyms THBD, Thrombomodulin, TM, THRM, Fetomodulin
Calculated Molecular Weight60 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberNM_000361.3
Gene Symbol THBD
Gene ID (NCBI) 7056
RRIDAB_3669952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

实验方案

Product Specific Protocols
WB protocol for CD141/Thrombomodulin antibody 31339-1-APDownload protocol
IF protocol for CD141/Thrombomodulin antibody 31339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols