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Conjugate
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验证数据展示

  • WB analysis using 31339-1-AP

    WB analysis using 31339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31339-1-AP (CD141/Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 31339-1-AP

    IF Staining of HUVEC using 31339-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CD141/Thrombomodulin antibody (31339-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

CD141/Thrombomodulin Polyclonal antibody

CD141/Thrombomodulin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31339-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

THBD, Thrombomodulin, TM, THRM, Fetomodulin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, THP-1 cells
Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD141/Thrombomodulin Ready-To-Use IHC Kit. CD141/Thrombomodulin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31339-1-AP targets CD141/Thrombomodulin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CD141/Thrombomodulin fusion protein Eg0944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thrombomodulin
别名 THBD, Thrombomodulin, TM, THRM, Fetomodulin
计算分子量60 kDa
观测分子量90 kDa
GenBank蛋白编号NM_000361.3
基因名称 THBD
Gene ID (NCBI) 7056
RRIDAB_3669952
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07204
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

实验方案

Product Specific Protocols
WB protocol for CD141/Thrombomodulin antibody 31339-1-APDownload protocol
IF protocol for CD141/Thrombomodulin antibody 31339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols