Anti-Human CD146 (P1H12)
CD146 Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
human, mouse, rabbit, canine
Applications
IF/ICC, FC
Conjugate
Unconjugated
CloneNo.
P1H12
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HUVEC cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CD146/MCAM Ready-To-Use IHC Kit. CD146/MCAM primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| FC | See 2 publications below |
产品信息
65181-1-Ig targets CD146 in IF/ICC, FC applications and shows reactivity with human, mouse, rabbit, canine samples.
| Tested Applications | IF/ICC, FC Application Description |
| Cited Applications | FC |
| Tested Reactivity | human, mouse, rabbit, canine |
| Cited Reactivity | human |
| Immunogen | cultured human umbilical cells 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | melanoma cell adhesion molecule |
| Synonyms | S-endo 1 endothelial-associated antigen, P1H12, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32 |
| Calculated Molecular Weight | 646 aa, 72 kDa |
| GenBank Accession Number | BC056418 |
| Gene Symbol | CD146 |
| Gene ID (NCBI) | 4162 |
| RRID | AB_2881483 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Purified by protein-A affinity chromatography |
| UNIPROT ID | P43121 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CD146 antibody 65181-1-Ig | Download protocol |
| FC protocol for CD146 antibody 65181-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction. | ||
Stem Cell Res Ther Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models |
