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验证数据展示

  • FC experiment of A375 using CL488-65181

    FC experiment of A375 using CL488-65181

    1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD146 (CL488-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

  • FC experiment of A375 using CL488-65181

    FC experiment of A375 using CL488-65181

    1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD146 (CL488-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

CoraLite® Plus 488 Anti-Human CD146 (P1H12)

CD146 Monoclonal Antibody for FC
Cat No. CL488-65181

产品说明书

CloneNo. P1H12

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Rabbit, Canine, Mouse, Human

应用

FC

别名

CD146, MCAM, MUC18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy CD146/MCAM Ready-To-Use IHC Kit. CD146/MCAM primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65181-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65181 targets CD146 in FC applications and shows reactivity with Rabbit, Canine, Mouse, Human samples.

经测试应用 FC Application Description
经测试反应性 Rabbit, Canine, Mouse, Human
免疫原 cultured human umbilical cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 melanoma cell adhesion molecule
别名 CD146, MCAM, MUC18
计算分子量 646 aa, 72 kDa
GenBank蛋白编号BC056418
基因名称 CD146
Gene ID (NCBI) 4162
RRIDAB_3084160
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP43121
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD146 antibody CL488-65181Download protocol
Standard Protocols
Click here to view our Standard Protocols