CoraLite®555-conjugated CD146/MCAM Monoclonal antibody
CD146/MCAM Monoclonal Antibody for IF/ICC, IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC, IF-P
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
4D8A9
验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | HUVEC cells |
Planning an IHC experiment? We recommend our IHCeasy CD146/MCAM Ready-To-Use IHC Kit. CD146/MCAM primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66153-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-66153 targets CD146/MCAM in IF/ICC, IF-P applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IF-P Application Description |
| Tested Reactivity | human |
| Immunogen | CD146/MCAM fusion protein Ag11855 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | melanoma cell adhesion molecule |
| Synonyms | CD146, 4D8A9, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32 |
| Calculated Molecular Weight | 646 aa, 72 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC056418 |
| Gene Symbol | CD146 |
| Gene ID (NCBI) | 4162 |
| RRID | AB_2919675 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P43121 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 CD146/MCAM antibody CL555-66153 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |