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CD147 Recombinant antibody

CD147 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230046C1

Cat no : 82909-1-RR

Print datasheet

Synonyms

EMMPRIN, Collagenase stimulatory factor, BSG, Basigin, 5F7

验证数据展示

  • IF Staining of HeLa using 82909-1-RR

    IF Staining of HeLa using 82909-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (82909-1-RR, Clone: 230046C1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • IF Staining of MCF-7 using 82909-1-RR

    IF Staining of MCF-7 using 82909-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CD147 antibody (82909-1-RR, Clone: 230046C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IF/ICC detected inHeLa cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CD147/BSG Ready-To-Use IHC Kit. CD147/BSG primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82909-1-RR targets CD147 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name basigin (Ok blood group)
Synonyms EMMPRIN, Collagenase stimulatory factor, BSG, Basigin, 5F7
Calculated Molecular Weight 385 aa, 42 kDa
GenBank Accession NumberBC009040
Gene Symbol CD147
Gene ID (NCBI) 682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35613
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).

实验方案

Product Specific Protocols
IF protocol for CD147 antibody 82909-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols