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验证数据展示

  • FC experiment of U-937 using AB-65251

    FC experiment of U-937 using AB-65251

    U-937 cells (positive) and Jurkat cells (negative) were mixed 1:1 and 1x10^6 cells were surface stained with 5 ul Atlantic Blue™ Anti-Human CD155/PVR (AB-65251, Clone: SKII.4) (red) or Atlantic Blue™ Mouse IgG1 Isotype Control (AB-65124, Clone: MOPC-21) (blue). Cells were not fixed.

  • FC experiment of U-937 using AB-65251

    FC experiment of U-937 using AB-65251

    U-937 cells (positive) and Jurkat cells (negative) were mixed 1:1 and 1x10^6 cells were surface stained with 5 ul Atlantic Blue™ Anti-Human CD155/PVR (AB-65251, Clone: SKII.4) or Atlantic Blue™ Mouse IgG1 Isotype Control (AB-65124, Clone: MOPC-21). Cells were not fixed.

Atlantic Blue™ Anti-Human CD155/PVR (SKII.4)

CD155/PVR Monoclonal Antibody for FC
Cat No. AB-65251

产品说明书

CloneNo. SKII.4

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC

别名

PVR, CD155, HVED, Necl 5, NECL5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Atlantic Blue™
规格: 

-/ -

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经过测试的应用

Positive FC detected inU-937 cells
Planning an IHC experiment? We recommend our IHCeasy PVR Ready-To-Use IHC Kit. PVR primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

AB-65251 targets CD155/PVR in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 SK-N-AS human neuroblastoma cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 poliovirus receptor
别名 PVR, CD155, HVED, Necl 5, NECL5
计算分子量 45 kDa
GenBank蛋白编号BC015542
基因名称 CD155/PVR
Gene ID (NCBI) 5817
偶联类型 Atlantic Blue™ Fluorescent Dye
最大激发/发射波长404 nm / 458 nm
形式 Liquid
纯化方式N/A
UNIPROT IDP15151
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.

实验方案

Product Specific Protocols
FC protocol for Atlantic Blue™ CD155/PVR antibody AB-65251Download protocol
Standard Protocols
Click here to view our Standard Protocols