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Species Reactivity
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验证数据展示

  • FC experiment of human peripheral blood leukocyte using 65612-1-MR (same clone as 65612-1-PBS)

    FC experiment of human peripheral blood leukocyte using 65612-1-MR (same clone as 65612-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16 Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone: 3G8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65612-1-PBS in a different storage buffer formulation.

  • FC experiment of human peripheral blood leukocyte using 65612-1-MR (same clone as 65612-1-PBS)

    FC experiment of human peripheral blood leukocyte using 65612-1-MR (same clone as 65612-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16 Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone:3G8) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65612-1-PBS in a different storage buffer formulation.

Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody, PBS Only

CD16 Recombinant Antibody for FC
Cat No. 65612-1-PBS

产品说明书

CloneNo. 3G8

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

产品信息

65612-1-PBS targets CD16 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A
计算分子量 254 aa, 29 kDa
GenBank蛋白编号BC017865
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).