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验证数据展示

  • FC experiment of human peripheral blood leukocyte using 65612-1-MR

    FC experiment of human peripheral blood leukocyte using 65612-1-MR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16 Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone: 3G8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocyte using 65612-1-MR

    FC experiment of human peripheral blood leukocyte using 65612-1-MR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16 Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone:3G8) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody

CD16 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65612-1-MR

产品说明书

CloneNo. 3G8

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

FC

别名

CD16, CD16A, CD16a antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65612-1-MR targets CD16 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 CD16, CD16A, CD16a antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2
计算分子量 254 aa, 29 kDa
GenBank蛋白编号BC017865
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
RRIDAB_3670359
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

实验方案

Product Specific Protocols
FC protocol for CD16 antibody 65612-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols