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CoraLite® Plus 647 Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody

CD16 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3G8

Cat no : CL647-65612

Print datasheet

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

验证数据展示

  • FC experiment of human peripheral blood leukocytes using CL647-65612

    FC experiment of human peripheral blood leukocytes using CL647-65612

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD16 Mouse IgG2a Recombinant Antibody (CL647-65612, Clone: 3G8), or CoraLite® Plus 647 Mouse IgG2a Isotype Control (CL647-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using CL647-65612

    FC experiment of human peripheral blood leukocytes using CL647-65612

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD16 Mouse IgG2a Recombinant Antibody (CL647-65612, Clone: 3G8) (red), or CoraLite® Plus 647 Mouse IgG2a Isotype Control (CL647-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65612-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65612 targets CD16 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Synonyms Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A
Calculated Molecular Weight 254 aa, 29 kDa
GenBank Accession NumberBC017865
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD16 antibody CL647-65612Download protocol
Standard Protocols
Click here to view our Standard Protocols