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Biotin Plus Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody

CD16 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Biotin Plus

CloneNo.

3G8

Cat no : Biotin-65612

Print datasheet

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

验证数据展示

  • FC experiment of human PBMCs using Biotin-65612

    FC experiment of human PBMCs using Biotin-65612

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Plus Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (Biotin-65612, Clone: 3G8), or unstained. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using Biotin-65612

    FC experiment of human PBMCs using Biotin-65612

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Plus Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (Biotin-65612, Clone: 3G8) (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-65612 targets CD16 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Synonyms Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A
Calculated Molecular Weight 254 aa, 29 kDa
GenBank Accession NumberBC017865
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
Conjugate Biotin Plus
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

实验方案

Product Specific Protocols
FC protocol for Biotin Plus CD16 antibody Biotin-65612Download protocol
Standard Protocols
Click here to view our Standard Protocols