FcZero-rAb™ APC Anti-Human CD16 (3G8) Rabbit IgG Recombinant Antibody

CD16 FcZero-rAbTM Recombinant Antibody for FC
Cat No. APC-FcA65612

产品说明书

CloneNo. 3G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  APC
规格: 

-/ -


经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-FcA65612 targets CD16 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A
计算分子量 254 aa, 29 kDa
GenBank蛋白编号BC017865
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
偶联类型 APC Fluorescent Dye
最大激发/发射波长650 nm / 660 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

实验方案

Product Specific Protocols
FC protocol for APC CD16 antibody APC-FcA65612Download protocol
Standard Protocols
Click here to view our Standard Protocols
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