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验证数据展示

  • FC experiment of human PBMCs using CL594-65090

    FC experiment of human PBMCs using CL594-65090

    1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 (CL750-65151) and 5 ul CoraLite®594 Anti-Human CD16 (CL594-65090, Clone:3G8) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL594-65090

    FC experiment of human PBMCs using CL594-65090

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD16 (CL594-65090, Clone:3G8) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124. Cells were not fixed. Lymphocytes were gated.

CoraLite®594 Anti-Human CD16 (3G8)

CD16 Monoclonal Antibody for FC
Cat No. CL594-65090

Print datasheet

CloneNo. 3G8

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65090-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65090 targets CD16 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Synonyms Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A
Calculated Molecular Weight 254 aa, 29 kDa
GenBank Accession NumberBC017865
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
RRIDAB_3673440
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

实验方案

Product Specific Protocols
FC protocol for CL594 CD16 antibody CL594-65090Download protocol
Standard Protocols
Click here to view our Standard Protocols