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验证数据展示

  • FC experiment of human PBMCs using CL594-65090

    FC experiment of human PBMCs using CL594-65090

    1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 (CL750-65151) and 5 ul CoraLite®594 Anti-Human CD16 (CL594-65090, Clone:3G8) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL594-65090

    FC experiment of human PBMCs using CL594-65090

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD16 (CL594-65090, Clone:3G8) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124. Cells were not fixed. Lymphocytes were gated.

CoraLite®594 Anti-Human CD16 (3G8)

CD16 Monoclonal Antibody for FC
Cat No. CL594-65090

产品说明书

CloneNo. 3G8

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC

别名

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65090-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65090 targets CD16 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A
计算分子量 254 aa, 29 kDa
GenBank蛋白编号BC017865
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
RRIDAB_3673440
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

实验方案

Product Specific Protocols
FC protocol for CL594 CD16 antibody CL594-65090Download protocol
Standard Protocols
Click here to view our Standard Protocols