PE Anti-Human CD16 (3G8)
CD16 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
human
Applications
FC
Conjugate
PE Fluorescent Dye
CloneNo.
3G8
验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.
推荐稀释比
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
FC | See 1 publications below |
产品信息
PE-65090 targets CD16 in FC applications and shows reactivity with human samples.
Tested Applications | FC Application Description |
Cited Applications | FC |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | Human PMN cells 种属同源性预测 |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
Synonyms | Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A |
Calculated Molecular Weight | 254 aa, 29 kDa |
GenBank Accession Number | BC017865 |
Gene Symbol | CD16a |
Gene ID (NCBI) | 2214 |
ENSEMBL Gene ID | ENSG00000203747 |
RRID | AB_2883884 |
Conjugate | PE Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Purification Method | Affinity purification |
UNIPROT ID | P08637 |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).
实验方案
Product Specific Protocols | |
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FC protocol for PE CD16 antibody PE-65090 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |