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- KD/KO Validated
CD163 Polyclonal antibody
CD163 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig and More (1)
Applications
WB, IHC, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
174
验证数据展示
经过测试的应用
Positive WB detected in | pig spleen tissue, human placenta tissue |
Positive IP detected in | human placenta tissue |
Positive IHC detected in | human tonsillitis tissue, human liver tissue, human placenta tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue, human liver tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16646-1-AP targets CD163 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, pig samples.
Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, pig |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | CD163 fusion protein Ag10029 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD163 molecule |
Synonyms | sCD163, Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor |
Calculated Molecular Weight | 1156 aa, 125 kDa |
Observed Molecular Weight | 130-150 kDa |
GenBank Accession Number | BC051281 |
Gene Symbol | CD163 |
Gene ID (NCBI) | 9332 |
ENSEMBL Gene ID | ENSG00000177575 |
RRID | AB_2756528 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q86VB7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD163 antibody 16646-1-AP | Download protocol |
IHC protocol for CD163 antibody 16646-1-AP | Download protocol |
IF protocol for CD163 antibody 16646-1-AP | Download protocol |
IP protocol for CD163 antibody 16646-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease | ||
J Clin Invest CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages | ||
Adv Sci (Weinh) Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis | ||
Cell Rep Med Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response | ||
Nat Commun Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target. | ||
Redox Biol Lactate-induced activation of tumor-associated fibroblasts and IL-8-mediated macrophage recruitment promote lung cancer progression |