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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CD163 Polyclonal antibody

CD163 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16646

Print datasheet

Synonyms

sCD163, Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor

验证数据展示

  • IF Staining of human liver using CL488-16646

    IF Staining of human liver using CL488-16646

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using CoraLite® Plus 488 CD163 antibody (CL488-16646, green) at dilution of 1:200, CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, magenta), ASGR1 antibody (11739-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA502, orange). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using CL488-16646

    IF Staining of human tonsillitis using CL488-16646

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD163 antibody (CL488-16646) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL488-16646

    IF Staining of human tonsillitis using CL488-16646

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD163 antibody (CL488-16646) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue, human liver cancer tissue, human liver tissue
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16646-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16646 targets CD163 in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen CD163 fusion protein Ag10029 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD163 molecule
Synonyms sCD163, Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor
Calculated Molecular Weight 1156 aa, 125 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
RRIDAB_2919139
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VB7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD163 antibody CL488-16646Download protocol
Standard Protocols
Click here to view our Standard Protocols