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验证数据展示

  • FC experiment of human PBMCs using CL647-65501

    FC experiment of human PBMCs using CL647-65501

    1x10^6 human PBMCs were surface stained with FITC Anti-Human CD14 and 5 ul CoraLite® Plus 647 Anti-Human CD163 Rabbit Recombinant Antibody (CL647-65501, Clone:GHI/61) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • FC experiment of human PBMCs using CL647-65501

    FC experiment of human PBMCs using CL647-65501

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD163 Rabbit Recombinant Antibody (CL647-65501, Clone:GHI/61) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

CoraLite® Plus 647 Anti-Human CD163 (GHI/61) Rabbit IgG Recombinant Antibody

CD163 Recombinant Antibody for FC
Cat No. CL647-65501

Print datasheet

CloneNo. GHI/61

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 
25tests
100tests

¥1860/ 100tests

浓度: 5 ul/test

库存：现货

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65501-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65501 targets CD163 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Glycoprotein preparation from hairy cell leukemia spleen 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD163 molecule
Synonyms Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61
Calculated Molecular Weight 1156 aa, 125 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
RRIDAB_3673580
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD163 antibody CL647-65501Download protocol
Standard Protocols
Click here to view our Standard Protocols