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FITC Plus Anti-Human CD163 (GHI/61) Mouse IgG1 Recombinant Antibody

CD163 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

GHI/61

Cat no : FITC-65522

Print datasheet

Synonyms

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61

验证数据展示

  • FC experiment of human PBMCs using FITC-65522

    FC experiment of human PBMCs using FITC-65522

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD163 Mouse Recombinant Antibody (FITC-65522, Clone: GHI/61) or FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21), and 5 ul APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65522 targets CD163 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Glycoprotein preparation from hairy cell leukemia spleen 种属同源性预测
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Full Name CD163 molecule
Synonyms Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61
Calculated Molecular Weight 1156 aa, 125 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD163 antibody FITC-65522Download protocol
Standard Protocols
Click here to view our Standard Protocols