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CD177 Polyclonal antibody

CD177 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18264-1-AP

Print datasheet

Synonyms

CD177, CD177 antigen, CD177 molecule, HNA 2a, HNA2A, NB1, NB1 glycoprotein, NB1 GP, PRV 1, PRV1

验证数据展示

  • WB analysis of mouse bone marrow using 18264-1-AP

    WB analysis of mouse bone marrow using 18264-1-AP

    mouse bone marrow tissue was subjected to SDS PAGE followed by western blot with 18264-1-AP (CD177 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse bone marrow

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18264-1-AP targets CD177 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CD177 fusion protein Ag13146 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD177 molecule
Synonyms CD177, CD177 antigen, CD177 molecule, HNA 2a, HNA2A, NB1, NB1 glycoprotein, NB1 GP, PRV 1, PRV1
Calculated Molecular Weight 437 aa, 46 kDa
Observed Molecular Weight100-130 kDa
GenBank Accession NumberBC029167
Gene Symbol CD177
Gene ID (NCBI) 57126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6Q3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD177, also known as NB1, HNA-2a or PRV-1, is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that belongs to the uPAR/CD59/Ly6 snake toxin superfamily (PMID: 17898576). It is expressed exclusively by neutrophils, neutrophilic metamyelocytes, and myelocytes (PMID: 17580308). CD177 interacts with β2 integrin CD11b/CD18 and CD11a/CD18, PECAM-1, and participates in neutrophil activation and transmigration (PMID: 17580308; 21193407). CD177 has been identified as a useful biomarker for myeloproliferative diseases (PMID: 17898576).

实验方案

Product Specific Protocols
WB protocol for CD177 antibody 18264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols