FcZero-rAb™ PE Anti-Human CD177 Rabbit Recombinant Antibody

CD177 FcZero-rAbTM Recombinant Antibody for FC
Cat No. PE-FcA98086
CloneNo. 241339F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

NB1, Human neutrophil alloantigen 2a, HNA-2a, HNA2A, HNA 2a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size: 

-/ -


经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-FcA98086 targets CD177 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD177 molecule
Synonyms NB1, Human neutrophil alloantigen 2a, HNA-2a, HNA2A, HNA 2a
Calculated Molecular Weight 437 aa, 46 kDa
GenBank Accession NumberBC029167
Gene Symbol CD177
Gene ID (NCBI) 57126
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD177, also known as NB1, HNA-2a or PRV-1, is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that belongs to the uPAR/CD59/Ly6 snake toxin superfamily (PMID: 17898576). It is expressed exclusively by neutrophils, neutrophilic metamyelocytes, and myelocytes (PMID: 17580308). CD177 interacts with β2 integrin CD11b/CD18 and CD11a/CD18, PECAM-1, and participates in neutrophil activation and transmigration (PMID: 17580308; 21193407). CD177 has been identified as a useful biomarker for myeloproliferative diseases (PMID: 17898576).

实验方案

Product Specific Protocols
FC protocol for PE CD177 antibody PE-FcA98086Download protocol
Standard Protocols
Click here to view our Standard Protocols
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