验证数据展示
经过测试的应用
| Positive FC detected in | Transfected HEK-293T cells |
For other applications, we recommend the unconjugated version of this antibody, 65598-1-MR
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-65598 targets CD178 (Fas ligand) in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
L5178Y mouse T lymphoma cells expressing recombinant human FasL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Fas ligand (TNF superfamily, member 6) |
| 别名 | CD178, Fas ligand, FASLG, ADAM10-processed FasL form, Apoptosis antigen ligand |
| 计算分子量 | 281 aa, 31 kDa |
| GenBank蛋白编号 | BC017502 |
| 基因名称 | Fas Ligand |
| Gene ID (NCBI) | 356 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P48023 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD178, also known as Fas ligand, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. It is expressed on NK cells, cytotoxic T lymphocytes and activated CD4+ Th1 cells. CD178 transduces apoptotic signal into cells by binding to FAS/CD95. It is involved as a death factor in the regulation of activation-induced cell death, establishment of immune privilege and tumor cell survival.
