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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65595-1-MR

    FC experiment of human PBMCs using 65595-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody (65595-1-MR, Clone: TS1/18) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD45 (HI30) (APC-65109, Clone: HI30). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 65595-1-MR

    FC experiment of human PBMCs using 65595-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody (65595-1-MR, Clone: TS1/18) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody

CD18 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65595-1-MR
CloneNo. TS1/18

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

ITGB2, LCAMB, LAD, Integrin beta-2, Integrin beta 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65595-1-MR targets CD18 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human Beta 2 Integrin 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Synonyms ITGB2, LCAMB, LAD, Integrin beta-2, Integrin beta 2
Calculated Molecular Weight 85 kDa
GenBank Accession NumberBC005861
Gene Symbol CD18
Gene ID (NCBI) 3689
ENSEMBL Gene IDENSG00000160255
RRIDAB_3670343
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

实验方案

Product Specific Protocols
FC protocol for CD18 antibody 65595-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols