• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 66257-1-Ig

    WB analysis of Jurkat using 66257-1-Ig

    WB result of CD1D antibody (66257-1-Ig, 1:500) with si-Control and si-CD1D transfected Jurkat cells.

  • WB analysis of human testis using 66257-1-Ig

    WB analysis of human testis using 66257-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66257-1-Ig (CD1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 66257-1-Ig

    IHC staining of human skin using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 66257-1-Ig

    IHC staining of human skin using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 40x lens).

  • Featured Product
  • KD/KO验证

CD1d Monoclonal antibody

CD1d Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66257-1-Ig

产品说明书

CloneNo. 1C12F1
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA and More (1)

别名

CD1A, CD1D, CD1d molecule, R3, R3G1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman testis tissue, Jurkat
Positive IHC detected inhuman skin tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66257-1-Ig targets CD1d in WB, IHC, ELISA, IF applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 CD1d fusion protein Ag11108 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD1d molecule
别名 CD1A, CD1D, CD1d molecule, R3, R3G1
计算分子量 335 aa, 38 kDa
观测分子量 40 kDa, 50-55 kDa
GenBank蛋白编号BC027926
基因名称 CD1D
Gene ID (NCBI) 912
RRIDAB_2881645
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP15813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-55 kDa.

实验方案

Product Specific Protocols
WB protocol for CD1d antibody 66257-1-IgDownload protocol
IHC protocol for CD1d antibody 66257-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Open Med (Wars)

CD1d affects the proliferation, migration, and apoptosis of human papillary thyroid carcinoma TPC-1 cells via regulating MAPK/NF-κB signaling pathway

Authors - Qingyuan Liu
  • KD Validated
View Article