验证数据展示
经过测试的应用
| Positive WB detected in | MOLT-4 cells, Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 66257-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66257 targets CD1d in WB applications and shows reactivity with human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human |
| 免疫原 | CD1d fusion protein Ag11108 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD1d molecule |
| 别名 | CD1A, CD1D, CD1d molecule, R3, R3G1 |
| 计算分子量 | 335 aa, 38 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC027926 |
| 基因名称 | CD1D |
| Gene ID (NCBI) | 912 |
| RRID | AB_3084226 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P15813 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-55 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 CD1d antibody CL488-66257 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |