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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CD1d Monoclonal antibody

CD1d Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C12F1

Cat no : CL488-66257

Print datasheet

Synonyms

CD1A, CD1D, CD1d molecule, R3, R3G1

验证数据展示

  • WB analysis using CL488-66257

    WB analysis using CL488-66257

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66257 (CD1d antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMOLT-4 cells, Jurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66257-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66257 targets CD1d in WB applications and shows reactivity with human samples.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen CD1d fusion protein Ag11108 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD1d molecule
Synonyms CD1A, CD1D, CD1d molecule, R3, R3G1
Calculated Molecular Weight 335 aa, 38 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC027926
Gene Symbol CD1D
Gene ID (NCBI) 912
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15813
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-55 kDa.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 CD1d antibody CL488-66257Download protocol
Standard Protocols
Click here to view our Standard Protocols