验证数据展示
经过测试的应用
| Positive FC detected in | MOLT-4 cells |
For other applications, we recommend the unconjugated version of this antibody, 65172-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65172 targets CD1a in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 | Human thymocytes 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD1a molecule |
| 别名 | hTa1 thymocyte antigen, HTA1, HI149, FCB6, CD1a molecule |
| 计算分子量 | 327 aa, 37 kDa |
| GenBank蛋白编号 | BC031645 |
| 基因名称 | CD1A |
| Gene ID (NCBI) | 909 |
| RRID | AB_3672868 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Unknow |
| UNIPROT ID | P06126 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD1a antibody CL488-65172 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |