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Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only

CD2 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS1/8

Cat no : 65565-1-PBS

Print datasheet

Synonyms

LFA-3 receptor, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor

验证数据展示

  • FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)

    FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone:TS1/8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65565-1-PBS

    FC experiment of human PBMCs using 65565-1-PBS

    1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) , or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with FITC Plus Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65565-1-PBS targets CD2 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD2 molecule
Synonyms LFA-3 receptor, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor
Calculated Molecular Weight 351 aa, 39 kDa
GenBank Accession NumberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
FC protocol for CD2 antibody 65565-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols