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验证数据展示

  • IF Staining of Jurkat using 84108-3-RR (same clone as 84108-3-PBS)

    IF Staining of Jurkat using 84108-3-RR (same clone as 84108-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD2 antibody (84108-3-RR, Clone: 241222E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84108-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01045-4

    Sandwich ELISA standard curve of MP01045-4

    Sandwich ELISA standard curve of MP01045-4, Human CD2 Recombinant Matched Antibody Pair - PBS only. 84108-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1271. 84108-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • Sample test of MP01045-4

    Sample test of MP01045-4

    Jurkat and Molt-4 cell lysate were measured. The human CD2 concentration of detected samples was determined to be 8,318.1 pg/mL (based on a 1.0 mg/mL extract load) in Jurkat cell lysate and 679.3 pg/mL (based on a 4.5 mg/mL extract load) in Molt-4 cell lysate.

  • Sample test of MP01045-4

    Sample test of MP01045-4

    Serum of six individual healthy human donors was measured. The human CD2 concentration of detected samples was determined to be 167.0 pg/mL with a range of 119.9 - 223.9 pg/mL.

  • Cytometric bead array standard curve of MP01045-2

    Cytometric bead array standard curve of MP01045-2

    Cytometric bead array standard curve of MP01045-2, CD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84108-2-PBS. Detection antibody: 84108-3-PBS. Standard: Eg1271. Range: 0.625-40 ng/mL.

  • Cytometric bead array standard curve of MP01045-3

    Cytometric bead array standard curve of MP01045-3

    Cytometric bead array standard curve of MP01045-3, CD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84108-1-PBS. Detection antibody: 84108-3-PBS. Standard: Eg1271. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 84108-3-RR

    Affinity and Kinetic Characterization of 84108-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84108-3-RR against Human CD2 were performed. The affinity constant is 4.93 nM.

CD2 Recombinant antibody, PBS Only (Detector)

CD2 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84108-3-PBS

产品说明书

CloneNo. 241222E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84108-3-PBS targets CD2 as part of a matched antibody pair:

MP01045-2: 84108-2-PBS capture and 84108-3-PBS detection (validated in Cytometric bead array)

MP01045-3: 84108-1-PBS capture and 84108-3-PBS detection (validated in Cytometric bead array)

MP01045-4: 84108-5-PBS capture and 84108-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD2 molecule
别名 LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule
计算分子量 351 aa, 39 kDa
GenBank蛋白编号BC033583
基因名称 CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06729
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.