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CD200 Monoclonal antibody

CD200 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66282-1-Ig

产品说明书

CloneNo. 5F3D6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA and More (1)

CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected infetal human brain tissue, human spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66282-1-Ig targets CD200 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human
文献引用反应性human
免疫原 CD200 fusion protein Ag5045 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD200 molecule
别名 CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein
计算分子量 269 aa, 30 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC022522
基因名称 CD200
Gene ID (NCBI) 4345
RRIDAB_2881665
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP41217
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

实验方案

Product Specific Protocols
WB protocol for CD200 antibody 66282-1-IgDownload protocol
IHC protocol for CD200 antibody 66282-1-IgDownload protocol
FC protocol for CD200 antibody 66282-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Rep

Inflammatory dendritic cells restrain CD11b+CD4+ CTLs via CD200R in human NSCLC

Authors - Mingjie Lin
humanIF

Cancers (Basel)

Multiplex Quantitative Analysis of Tumor-Infiltrating Lymphocytes, Cancer-Associated Fibroblasts, and CD200 in Pancreatic Cancer.

Authors - Tyler MacNeil
humanWB,IHC

Sci Rep

CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors.

Authors - Masayuki Ishibashi
  • KD Validated
humanWB,IHC,IF

Oncoimmunology

CD200 and CD200R1 are differentially expressed and have differential prognostic roles in non-small cell lung cancer.

Authors - Katsuhiro Yoshimura
  • KD Validated
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