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  • Featured Product
  • KD/KO Validated

CD200 Monoclonal antibody, PBS Only

CD200 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5F3D6

Cat no : 66282-1-PBS

Print datasheet

Synonyms

CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein

验证数据展示

  • WB analysis of fetal human brain using 66282-1-Ig (same clone as 66282-1-PBS)

    WB analysis of fetal human brain using 66282-1-Ig (same clone as 66282-1-PBS)

    Untreated and PNGase F-treated lysates of fetal human brain tissue and human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

    IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

    IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

产品信息

66282-1-PBS targets CD200 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD200 fusion protein Ag5045 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD200 molecule
Synonyms CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein
Calculated Molecular Weight 269 aa, 30 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC022522
Gene Symbol CD200
Gene ID (NCBI) 4345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41217
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).