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CD200R1 Recombinant antibody

CD200R1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240979B6

Cat no : 83889-4-RR

Print datasheet

Synonyms

OX2R, Mox2r, CD200R, CD200 receptor 1, 240979B6

验证数据展示

  • WB analysis of RAW 264.7 using 83889-4-RR

    WB analysis of RAW 264.7 using 83889-4-RR

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83889-4-RR (CD200R1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83889-4-RR

    Affinity and Kinetic Characterization of 83889-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83889-4-RR against Mouse CD200R1 were performed. The affinity constant is 4.26 nM.

经过测试的应用

Positive WB detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83889-4-RR targets CD200R1 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD200 receptor 1
Synonyms OX2R, Mox2r, CD200R, CD200 receptor 1, 240979B6
Calculated Molecular Weight36 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberNM_021325.3
Gene Symbol Cd200r1
Gene ID (NCBI) 57781
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9ES57
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cell surface glycoprotein CD200 receptor 1 (CD200R1) is also called CD200R, CRTR2, MOX2R, and OX2R, and belongs to the CD200R family. CD200R1 is an inhibitory receptor for the CD200/OX2 cell surface glycoprotein. CD200R1 is highly glycosylated, containing ten potential N-glycosylation sites (PMID: 15187158). CD200R1 limits inflammation by inhibiting the expression of pro-inflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. As an immune inhibitory molecule, CD200R1 binds to CD200, ultimately leading to the inhibition of classical activation of macrophages and microglia (PMID: 24588829).

实验方案

Product Specific Protocols
WB protocol for CD200R1 antibody 83889-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols