验证数据展示
经过测试的应用
| Positive IF-P detected in | human lung cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 7 publications below |
产品信息
CL488-60143 targets CD206 in IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF-P Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mannose receptor, C type 1 |
| 别名 | hMR, C-type lectin domain family 13 member D-like, C-type lectin domain family 13 member D, CLEC13DL, CLEC13D |
| 计算分子量 | 166 kDa |
| GenBank蛋白编号 | NM_002438 |
| 基因名称 | CD206 |
| Gene ID (NCBI) | 4360 |
| RRID | AB_2883105 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P22897 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Background
CD206 (macrophage mannose receptor 1) is a lectin-type endocytic receptor expressed on selected macrophages, dendritic cells, and non-vascular endothelium and plays a role in antigen processing and presentation, phagocytosis, and intracellular signaling.
1. What is the molecular weight of CD206?
The molecular size of full-length CD206 is 170-180 kDa, depending on the exact tissue-specific glycosylation pattern (PMID: 19427834). Additionally, CD206 can be cleaved off and a soluble form (sMR) lacking the tail, with a slightly lower molecular weight, can be released to the cell medium (PMID: 9722572).
2. What is the subcellular localization of CD206?
CD206 is a type I membrane protein composed of a large extracellular multidomain, a transmembrane domain, and a short cytoplasmic tail. It is present at the plasma membrane and in endosomes, as CD206 undergoes constant recycling between the plasma membrane and endosomal compartment.
3. Is CD206 post-translationally modified?
CD206 undergoes quite extensive post-translational modifications, predominantly N-linked glycosylation that affects ligand binding recognition and affinity (PMID: 22966131).
4. Can CD206 marker be used as a marker of M2 macrophages?
The activation of macrophages with various stimuli leads to their polarization into classical (M1) or alternatively activated (M2) subtypes spectrums and both subtypes differ in their regulatory and effector functions (PMID: 24669294). Pathogens and IFN-γ promote M1 polarization, while IL-4 released during parasite infections and allergen response promotes M2 polarization. Classically, the markers of M2 macrophages include CD206, as well as arginase-1 (ARG1; https://www.ptglab.com/products/ARG1-Antibody-16001-1-AP.htm), CD163 (https://www.ptglab.com/products/CD163-Antibody-16646-1-AP.htm), and thrombospondin 1 (TSP1/ THBS1; https://www.ptglab.com/products/TSP1-Antibody-18304-1-AP.htm).
5. How can you polarize macrophages into M2 direction?
One of the most commonly used methods is stimulation by the addition of IL-4 cytokine. We recommend using our animal-free human IL-4 (https://www.ptglab.com/products/recombinant-human-il-4.htm).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD206 antibody CL488-60143 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Nanomedicine Unexpected Inhibitory Role of Silica Nanoparticles on Lung Cancer Development by Promoting M1 Polarization of Macrophages | ||
Acta Biochim Biophys Sin (Shanghai) 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction | ||
J Control Release Injectable hydrogel harnessing foreskin mesenchymal stem cell-derived extracellular vesicles for treatment of chronic diabetic skin wounds | ||
Theranostics Comprehensive multi-omics analysis of pyroptosis for optimizing neoadjuvant immunotherapy in patients with gastric cancer | ||
J Ethnopharmacol Erjingpill bionic cerebrospinal fluid alleviates LPS-induced inflammatory response in BV2 cells by inhibiting glycolysis via mTOR | ||
Cancer Gene Ther CDK5RAP3 acts as a tumour suppressor in gastric cancer through the infiltration and polarization of tumour-associated macrophages |