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Anti-Human CD21 (BU32) Mouse IgG2a Recombinant Antibody

CD21 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BU32

Cat no : 65591-1-MR

Print datasheet

Synonyms

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

验证数据展示

  • FC experiment of human PBMCs using 65591-1-MR

    FC experiment of human PBMCs using 65591-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD21 Mouse IgG2a Recombinant Antibody (65591-1-MR, Clone: BU32) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65591-1-MR

    FC experiment of human PBMCs using 65591-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD21 Mouse IgG2a Recombinant Antibody (65591-1-MR, Clone: BU32) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65591-1-MR targets CD21 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name complement component (3d/Epstein Barr virus) receptor 2
Synonyms C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9
Calculated Molecular Weight 1092 aa, 119 kDa
GenBank Accession NumberBC136394
Gene Symbol CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

实验方案

Product Specific Protocols
FC protocol for CD21 antibody 65591-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols