验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-65591 targets CD21 in FC applications and shows reactivity with human samples.
经测试应用 | FC Application Description |
经测试反应性 | human |
免疫原 | N/A 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | complement component (3d/Epstein Barr virus) receptor 2 |
别名 | EBV receptor, CR2, Complement receptor type 2, Complement C3d receptor, C3DR |
计算分子量 | 1092 aa, 119 kDa |
GenBank蛋白编号 | BC136394 |
基因名称 | CD21 |
Gene ID (NCBI) | 1380 |
ENSEMBL Gene ID | ENSG00000117322 |
偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
最大激发/发射波长 | 755 nm / 780 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 750 CD21 antibody CL750-65591 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |