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验证数据展示

  • WB analysis using 84265-5-RR (same clone as 84265-5-PBS)

    WB analysis using 84265-5-RR (same clone as 84265-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84265-5-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84265-5-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84265-5-RR (same clone as 84265-5-PBS)

    IHC staining of human tonsillitis using 84265-5-RR (same clone as 84265-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84265-5-RR (CD22 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84265-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD22 antibody included
  • IHC staining of human tonsillitis using 84265-5-RR (same clone as 84265-5-PBS)

    IHC staining of human tonsillitis using 84265-5-RR (same clone as 84265-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84265-5-RR (CD22 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84265-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD22 antibody included
  • Affinity and Kinetic Characterization of 84265-5-RR

    Affinity and Kinetic Characterization of 84265-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84265-5-RR against Human CD22 were performed. The affinity constant is 262 nM.

CD22 Recombinant antibody, PBS Only

CD22 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84265-5-PBS

产品说明书

CloneNo. 241314B5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.

产品信息

84265-5-PBS targets CD22 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD22 molecule
别名 CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22
计算分子量 847 aa, 95 kDa
观测分子量140 kDa
GenBank蛋白编号BC109306
基因名称 CD22
Gene ID (NCBI) 933
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP20273
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.