• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD22 Rabbit Recombinant Antibody, PBS Only

CD22 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241315A1

Cat no : 98092-1-PBS

Print datasheet

Synonyms

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

验证数据展示

  • FC experiment of human PBMCs using 98092-1-RR (same clone as 98092-1-PBS)

    FC experiment of human PBMCs using 98092-1-RR (same clone as 98092-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR, Clone:241315A1) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG, Cells were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. This data was developed using the same antibody clone with 98092-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98092-1-RR (same clone as 98092-1-PBS)

    FC experiment of human PBMCs using 98092-1-RR (same clone as 98092-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR, Clone:241315A1) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98092-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98092-1-RR

    Affinity and Kinetic Characterization of 98092-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98092-1-RR against Human CD22 were performed. The affinity constant is 3.11 nM.

Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.

产品信息

98092-1-PBS targets CD22 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD22 molecule
Synonyms CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22
Calculated Molecular Weight 847 aa, 95 kDa
GenBank Accession NumberBC109306
Gene Symbol CD22
Gene ID (NCBI) 933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP20273
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.