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CoraLite® Plus 405 Anti-Human CD226 (11A8) Mouse IgG2a Recombinant Antibody

CD226 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

11A8

Cat No : CL405-65573

Print datasheet

Synonyms

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

验证数据展示

  • FC experiment of human PBMCs using CL405-65573

    FC experiment of human PBMCs using CL405-65573

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul CoraLite® Plus 405 Anti-Human CD226 (11A8) Mouse IgG2a Recombinant Antibody (CL405-65573, Clone: 11A8) (right), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL405-65573

    FC experiment of human PBMCs using CL405-65573

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD226 (11A8) Mouse IgG2a Recombinant Antibody (CL405-65573, Clone: 11A8)(red), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65573-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65573 targets CD226 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human NK Cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD226 molecule
Synonyms DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen
Calculated Molecular Weight 336 aa, 39 kDa
GenBank Accession NumberBC074787
Gene Symbol CD226
Gene ID (NCBI) 10666
ENSEMBL Gene IDENSG00000150637
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission Maxima Wavelengths399 nm / 422 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

背景介绍

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 CD226 antibody CL405-65573Download protocol
Standard Protocols
Click here to view our Standard Protocols