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CD23 Recombinant antibody, PBS Only (Capture/Detector)

CD23 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241269C8

Cat no : 84024-3-PBS

Print datasheet

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

验证数据展示

  • Sandwich ELISA standard curve of MP00958-3

    Sandwich ELISA standard curve of MP00958-3

    Sandwich ELISA standard curve of MP00958-3, Human CD23 Recombinant Matched Antibody Pair - PBS only. 84024-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02343. 84024-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL

  • Sample test of MP00958-3

    Sample test of MP00958-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean CD23 concentration was determined to be 16.3 pg/mL in unstimulated PBMC supernatant, 444.73 pg/mL in PHA stimulated PBMC supernatant. Raji human Burkitt’s lymphoma cells were cultured in RPMI supplemented with 10% fetal bovine serum, 2mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean CD23 concentration was determined to be 742.6 pg/mL Raji cell culture supernatants.

  • Sample test of MP00958-3

    Sample test of MP00958-3

    Serum of sixteen individual healthy human donors was measured. The human CD23 concentration of detected samples was determined to be 651.0 pg/mL with a range of 259.5 - 1,165.0 pg/mL.

  • Cytometric bead array standard curve of MP00958-2

    Cytometric bead array standard curve of MP00958-2

    Cytometric bead array standard curve of MP00958-2, CD23 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84024-1-PBS. Detection antibody: 84024-3-PBS. Standard: RP02343. Range: 0.156-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.

产品信息

84024-3-PBS targets CD23 as part of a matched antibody pair:

MP00958-2: 84024-1-PBS capture and 84024-3-PBS detection (validated in Cytometric bead array)

MP00958-3: 84024-3-PBS capture and 84024-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2
Calculated Molecular Weight 321 aa, 36 kDa
GenBank Accession NumberBC064417
Gene Symbol CD23
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06734
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C