CD24 Monoclonal antibody
CD24 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1H5C4
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells, HL-60 cells |
Positive IF/ICC detected in | Daudi cells |
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
67627-1-Ig targets CD24 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | CD24 fusion protein Ag11679 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | CD24 molecule |
Synonyms | CD24A, 1H5C4 |
Calculated Molecular Weight | 8 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC007674 |
Gene Symbol | CD24 |
Gene ID (NCBI) | 100133941 |
RRID | AB_2882828 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P25063 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)
实验方案
Product Specific Protocols | |
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WB protocol for CD24 antibody 67627-1-Ig | Download protocol |
IF protocol for CD24 antibody 67627-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Breast Cancer (Dove Med Press) CD24 May Serve as an Immunotherapy Target in Triple-Negative Breast Cancer by Regulating the Expression of PD-L1 |