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CD24 Monoclonal antibody

CD24 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H5C4

Cat No : 67627-1-Ig

Print datasheet

Synonyms

CD24, CD24 molecule, CD24A, FLJ22950, FLJ43543, Signal transducer CD24

验证数据展示

  • WB analysis using 67627-1-Ig

    WB analysis using 67627-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of Ramos using 67627-1-Ig

    FC experiment of Ramos using 67627-1-Ig

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human CD24 (67627-1-Ig, Clone:1H5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inMCF-7 cells, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells, HL-60 cells
Positive FC detected inRamos cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67627-1-Ig targets CD24 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CD24 fusion protein Ag11679 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD24 molecule
Synonyms CD24, CD24 molecule, CD24A, FLJ22950, FLJ43543, Signal transducer CD24
Calculated Molecular Weight8 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC007674
Gene Symbol CD24
Gene ID (NCBI) 100133941
RRIDAB_2882828
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25063
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

实验方案

Product Specific Protocols
WB protocol for CD24 antibody 67627-1-IgDownload protocol
FC protocol for CD24 antibody 67627-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Breast Cancer (Dove Med Press)

CD24 May Serve as an Immunotherapy Target in Triple-Negative Breast Cancer by Regulating the Expression of PD-L1

Authors - Xudong Zhu
View Article