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CD24 Monoclonal antibody, PBS Only

CD24 Monoclonal Antibody for WB,FC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,FC,ELISA

Conjugate

Unconjugated

CloneNo.

1H5C4

Cat No : 67627-1-PBS

Print datasheet

Synonyms

CD24, CD24 molecule, CD24A, FLJ22950, FLJ43543, Signal transducer CD24

验证数据展示

  • WB analysis using 67627-1-Ig (same clone as 67627-1-PBS)

    WB analysis using 67627-1-Ig (same clone as 67627-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67627-1-PBS in a different storage buffer formulation.

  • FC experiment of Ramos using 67627-1-Ig (same clone as 67627-1-PBS)

    FC experiment of Ramos using 67627-1-Ig (same clone as 67627-1-PBS)

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human CD24 (67627-1-Ig, Clone:1H5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67627-1-PBS in a different storage buffer formulation.

产品信息

67627-1-PBS targets CD24 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB,FC,ELISA
Tested Reactivity Human
Immunogen CD24 fusion protein Ag11679 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD24 molecule
Synonyms CD24, CD24 molecule, CD24A, FLJ22950, FLJ43543, Signal transducer CD24
Calculated Molecular Weight8 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC007674
Gene Symbol CD24
Gene ID (NCBI) 100133941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25063
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)