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Anti-Human CD244 (2B4.69)

CD244 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

2B4.69

Cat no : 65270-1-Ig

Print datasheet

Synonyms

2B4, CD244, h2B4, NAIL, NKR2B4, SLAMF4

验证数据展示

  • FC experiment of human PBMCs using 65270-1-Ig

    FC experiment of human PBMCs using 65270-1-Ig

    1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD56 and 0.2 ug Anti-Human CD244 (65270-1-Ig, Clone:2B4.69) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65270-1-Ig

    FC experiment of human PBMCs using 65270-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD244 (65270-1-Ig, Clone:2B4.69) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65270-1-Ig targets CD244 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD244 molecule, natural killer cell receptor 2B4
Synonyms 2B4, CD244, h2B4, NAIL, NKR2B4, SLAMF4
GenBank Accession NumberBC028073
Gene Symbol CD244
Gene ID (NCBI) 51744
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage Conditions2-8°C.

背景介绍

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).

实验方案

Product Specific Protocols
FC protocol for CD244 antibody 65270-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols