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CoraLite® Plus 594 Anti-Human CD244 (2B4.69)

CD244 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

2B4.69

Cat no : CL594-65270

Print datasheet

Synonyms

2B4, CD244, h2B4, NAIL, NKR2B4, SLAMF4

验证数据展示

  • FC experiment of human PBMCs using CL594-65270

    FC experiment of human PBMCs using CL594-65270

    1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD56 and 5 ul CoraLite® Plus 594 Anti-Human CD244 (2B4.69) (CL594-65270) or CoraLite®594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL594-65270

    FC experiment of human PBMCs using CL594-65270

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD244 (2B4.69) (CL594-65270) or CoraLite®594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65270-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65270 targets CD244 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD244 molecule, natural killer cell receptor 2B4
Synonyms 2B4, CD244, h2B4, NAIL, NKR2B4, SLAMF4
GenBank Accession NumberBC028073
Gene Symbol CD244
Gene ID (NCBI) 51744
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths594 nm / 615 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).

实验方案

Product Specific Protocols
FC protocol for CL Plus 594 CD244 antibody CL594-65270Download protocol
Standard Protocols
Click here to view our Standard Protocols