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Anti-Human CD25 (BC96) Mouse IgG2a Recombinant Antibody

CD25 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BC96

Cat no : 65620-1-MR

Print datasheet

Synonyms

IL-2 receptor subunit alpha, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha, IL 2 RA

验证数据展示

  • FC experiment of human PBMCs using 65620-1-MR

    FC experiment of human PBMCs using 65620-1-MR

    1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human CD25 (BC96) Mouse IgG2a Recombinant Antibody (65620-1-MR, Clone: BC96) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD25 (BC96) Mouse IgG2a Recombinant Antibody (65620-1-MR, Clone: BC96) and PE-conjugated Goat Anti-Mouse IgG (black, dashed). Cells were not fixed.

经过测试的应用

Positive FC detected inPHA treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65620-1-MR targets CD25 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name interleukin 2 receptor, alpha
Synonyms IL-2 receptor subunit alpha, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha, IL 2 RA
Calculated Molecular Weight 272 aa, 31 kDa
GenBank Accession NumberBC156627
Gene Symbol IL2RA
Gene ID (NCBI) 3559
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

实验方案

Product Specific Protocols
FC protocol for CD25 antibody 65620-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols