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Anti-Human CD25 (BC96) Mouse IgG2a Recombinant Antibody, PBS Only

CD25 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BC96

Cat no : 65620-1-PBS

Print datasheet

Synonyms

IL-2 receptor subunit alpha, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha, IL 2 RA

验证数据展示

  • FC experiment of human PBMCs using 65620-1-MR (same clone as 65620-1-PBS)

    FC experiment of human PBMCs using 65620-1-MR (same clone as 65620-1-PBS)

    1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human CD25 (BC96) Mouse IgG2a Recombinant Antibody (65620-1-MR, Clone: BC96) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD25 (BC96) Mouse IgG2a Recombinant Antibody (65620-1-MR, Clone: BC96) and PE-conjugated Goat Anti-Mouse IgG (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 65620-1-PBS in a different storage buffer formulation.

产品信息

65620-1-PBS targets CD25 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name interleukin 2 receptor, alpha
Synonyms IL-2 receptor subunit alpha, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha, IL 2 RA
Calculated Molecular Weight 272 aa, 31 kDa
GenBank Accession NumberBC156627
Gene Symbol IL2RA
Gene ID (NCBI) 3559
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).