• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD27 Recombinant antibody, PBS Only (Capture)

CD27 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242731G8

Cat no : 85066-3-PBS

Print datasheet

Synonyms

T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen

验证数据展示

  • Sandwich ELISA standard curve of MP01811-3

    Sandwich ELISA standard curve of MP01811-3

    Sandwich ELISA standard curve of MP01811-3, Human CD27 Recombinant Matched Antibody Pair - PBS only. 85066-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1644. 85066-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01811-3

    Sample test of MP01811-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean CD27 concentration was determined to be 1.61 ng/mL in unstimulated PBMC supernatant, 7.31 ng/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP01811-3

    Sample test of MP01811-3

    Serum of six individual healthy human donors was measured. The CD27 concentration of detected samples was determined to be 39.19 ng/mL with a range of 27.45-50.05 ng/mL.

  • Cytometric bead array standard curve of MP01811-2

    Cytometric bead array standard curve of MP01811-2

    Cytometric bead array standard curve of MP01811-2, CD27 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85066-3-PBS. Detection antibody: 85066-1-PBS. Standard: Eg1644. Range: 0.781-100 ng/mL.

产品信息

85066-3-PBS targets CD27 as part of a matched antibody pair:

MP01811-2: 85066-3-PBS capture and 85066-1-PBS detection (validated in Cytometric bead array)

MP01811-3: 85066-3-PBS capture and 85066-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD27 molecule
Synonyms T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen
Calculated Molecular Weight 29 kDa
GenBank Accession NumberBC012160
Gene Symbol CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26842
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C