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CoraLite® Plus 647 Anti-Human CD27 (O323)

CD27 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

O323

Cat no : CL647-65132

Print datasheet

Synonyms

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

验证数据展示

  • FC experiment of human peripheral blood lymphocytes using CL647-65132

    FC experiment of human peripheral blood lymphocytes using CL647-65132

    1X10^6 human peripheral blood lymphocytes were surface stained with 5.00 ul CoraLite® Plus 647-conjugated Anti-Human CD27 (CL647-65132, Clone: O323) (red) or isotype control antibody (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes
For other applications, we recommend the unconjugated version of this antibody, 65132-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65132 targets CD27 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD27 molecule
Synonyms CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55
Calculated Molecular Weight 29 kDa
GenBank Accession NumberBC012160
Gene Symbol CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
RRIDAB_2883699
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP26842
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD27 antibody CL647-65132Download protocol
Standard Protocols
Click here to view our Standard Protocols