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PE Anti-Mouse CD27 Rabbit Recombinant Antibody

CD27 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

241378E11

Cat no : PE-98160

Print datasheet

Synonyms

Tumor necrosis factor receptor superfamily member 7, Tnfrsf7, T-cell activation antigen CD27, CD27L receptor, CD27 antigen

验证数据展示

  • FC experiment of mouse splenocytes using PE-98160

    FC experiment of mouse splenocytes using PE-98160

    1x10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2), and 0.25 ug PE Anti-Mouse CD27 Rabbit RecAb (PE-98160, Clone:241378E11) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9). Cells were not fixed.

  • FC experiment of mouse splenocytes using PE-98160

    FC experiment of mouse splenocytes using PE-98160

    1x10^6 mouse splenocytes were surface stained with 0.25 ug PE Anti-Mouse CD27 Rabbit RecAb (PE-98160, Clone:241378E11) (red) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-98160 targets CD27 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD27 antigen
Synonyms Tumor necrosis factor receptor superfamily member 7, Tnfrsf7, T-cell activation antigen CD27, CD27L receptor, CD27 antigen
Calculated Molecular Weight28kDa
GenBank Accession NumberNM_001033126.2
Gene Symbol CD27
Gene ID (NCBI) 21940
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41272
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

实验方案

Product Specific Protocols
FC protocol for PE CD27 antibody PE-98160Download protocol
Standard Protocols
Click here to view our Standard Protocols