CoraLite® Plus 488 Anti-Mouse CD274 (PD-L1, B7-H1) (10F.9G2)
CD274 (PD-L1, B7-H1) Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
10F.9G2
验证数据展示
经过测试的应用
| Positive FC detected in | Anti-CD3/CD28 treated mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65073-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65073 targets CD274 (PD-L1, B7-H1) in FC applications and shows reactivity with Mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Mouse |
| Immunogen | N/A 种属同源性预测 |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD274 antigen |
| Synonyms | B7 H1, Cd274, CD274 (PD-L1, B7-H1), CD274 antigen, PD L1, Pdcd1l1, Pdcd1lg1 |
| GenBank Accession Number | BC066841 |
| Gene Symbol | Cd274 |
| Gene ID (NCBI) | 60533 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q9EP73 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD274 (PD-L1, B7-H1) antibody CL488-65073 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
