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CD276 Recombinant antibody, PBS Only (Capture)

CD276 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230305C1

Cat no : 83152-2-PBS

Print datasheet

Synonyms

B7-H3, B7H3, B7 homolog 3, B7 H3, 4Ig-B7-H3

验证数据展示

  • Sandwich ELISA standard curve of MP00248-3

    Sandwich ELISA standard curve of MP00248-3

    Sandwich ELISA standard curve of MP00248-3, Human B7-H3/CD276 Recombinant Matched Antibody Pair - PBS only. 83152-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0075. 83152-1-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

  • Sample test of MP00248-3

    Sample test of MP00248-3

    A549 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human B7-H3/CD276, and measured 346.1 pg/mL.

  • Sample test of MP00248-3

    Sample test of MP00248-3

    Urine of eight individual healthy human donors was measured. The B7-H3/CD276 concentration of detected samples was determined to be 19.6 ng/mL with a range of 3.0 - 52.0 ng/mL.

  • Sample test of MP00248-3

    Sample test of MP00248-3

    The mean B7-H3/CD276 concentration was determined to be 178.3 ng/mL in A549 cell extract based on a 3.8 mg/mL extract load.

  • Sample test of MP00248-3

    Sample test of MP00248-3

    Plasma of sixteen individual healthy human donors was measured. The B7-H3/CD276 concentration of detected samples was determined to be 12.9 ng/mL with a range of 6.4 - 21.2 ng/mL.

  • Cytometric bead array standard curve of MP00248-2

    Cytometric bead array standard curve of MP00248-2

    Cytometric bead array standard curve ofMP00248-2, CD276 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83152-2-PBS. Detection antibody: 83152-3-PBS. Standard: Eg0075. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00248-3

    Cytometric bead array standard curve of MP00248-3

    Cytometric bead array standard curve ofMP00248-3, CD276 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83152-2-PBS. Detection antibody: 83152-1-PBS. Standard: Eg0075. Range: 0.625-20 ng/mL.

产品信息

83152-2-PBS targets CD276 as part of a matched antibody pair:

MP00248-2: 83152-2-PBS capture and 83152-3-PBS detection (validated in Cytometric bead array)

MP00248-3: 83152-2-PBS capture and 83152-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD276 molecule
Synonyms B7-H3, B7H3, B7 homolog 3, B7 H3, 4Ig-B7-H3
Calculated Molecular Weight57 kDa
GenBank Accession NumberNM_001024736
Gene Symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5ZPR3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C