• 概述
  • 经过测试的应用
  • 产品信息
  • 实验方案
  • 相关产品 (1)

APC Anti-Human PD-1/CD279 (EH12.2H7)

PD-1/CD279 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Non-human primates, Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

EH12.2H7

Cat no : APC-65168

Print datasheet

Synonyms

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2



经过测试的应用

Positive FC detected inhuman peripheral blood mononuclear cells cells
Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65168 targets PD-1/CD279 in FC applications and shows reactivity with Non-human primates, Human samples.

Tested Applications FC Application Description
Tested Reactivity Non-human primates, Human
Immunogen Recombinant human CD279 protein 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2
Calculated Molecular Weight 288 aa, 32 kDa
GenBank Accession NumberBC074740
Gene Symbol PD-1
Gene ID (NCBI) 5133
RRIDAB_2883038
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDQ15116
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

实验方案

Product Specific Protocols
FC protocol for APC PD-1/CD279 antibody APC-65168Download protocol
Standard Protocols
Click here to view our Standard Protocols