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CoraLite® Plus 488 Anti-Human PD-1/CD279 (EH12.2H7)

PD-1/CD279 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Non-human primates, Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

EH12.2H7

Cat no : CL488-65168

Print datasheet

Synonyms

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

验证数据展示

  • FC experiment of human PBMCs using CL488-65168

    FC experiment of human PBMCs using CL488-65168

    1X10^6 PHA treated human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human PD-1/CD279 (CL488-65168, Clone:EH12.2H7) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21). Cells were not fixed.

  • FC experiment of human PBMCs using CL488-65168

    FC experiment of human PBMCs using CL488-65168

    1X10^6 day 3 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human PD-1/CD279 (CL488-65168, Clone: EH12.2H7) (red) or Mouse IgG1 Isotype Control (blue). Unactivated PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human PD-1/CD279 (black). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inPHA treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65168-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65168 targets PD-1/CD279 in FC applications and shows reactivity with Non-human primates, Human samples.

Tested Applications FC Application Description
Tested Reactivity Non-human primates, Human
Immunogen Recombinant human CD279 protein 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2
Calculated Molecular Weight 288 aa, 32 kDa
GenBank Accession NumberBC074740
Gene Symbol PD-1
Gene ID (NCBI) 5133
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ15116
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PD-1/CD279 antibody CL488-65168Download protocol
Standard Protocols
Click here to view our Standard Protocols